1443 - 1509 (66 years)
 | John Stewart 1531-1563 |
| John Stewart | |
| B: | 1 May 1531 |
| Edinburgh, Midlothian, Lothian, Scotland | |
| D: | 1 Nov 1563 |
| Inverness, Scotland | |
| Lady Jean Hepburn 1542-1599 |
| Lady Jean Hepburn | |
| B: | 1542 |
| Crichton Castle, Midlothian, Scotland | |
| D: | 27 Jul 1599 |
| Girnigo Castle, Caithness, Scotland | |
| James (James V) Stewart 1512-1542 |
| James (James V) Stewart | |
| B: | 15 Apr 1512 |
| Linlithgow, West Lothian, Scotland | |
| D: | 14 Dec 1542 |
| Falkland, Fife, Scotland | |
| Elizabeth Carmichel 1473-1559 |
| Elizabeth Carmichel | |
| B: | 1473 |
| Balmedie, Lanarkshire, Scotland | |
| D: | 15 Aug 1559 |
| Carnwath, Lanarkshire, Scotland | |
| Mary "Queen of Scots" Stewart 1542-1587 |
| Mary "Queen of Scots" Stewart | |
| B: | 8 Dec 1542 |
| Linlithgow Palace, Linlithgow, Linlithgowshire, Scotland | |
| D: | 8 Feb 1587 |
| Fotheringhay, Northamptonshire, England | |
| Duke Of Albany And King Consort Of Scotland Henry Stuart | |
| B: | 7 Dec 1545 |
| Temple Newson, Yorkshire, England | |
| M: | 29 Jul 1565 |
| Holyrood, Scotland | |
| D: | 10 Feb 1567 |
| Kirk O'Field, Edinburgh, MidLothian, Scotland | |
| Marie Guise 1515-1560 |
| Marie Guise | |
| B: | 22 Nov 1515 |
| Bar-le-Duc, Duché de Bar, Saint-Empire Romain Germanique | |
| M: | 12 Jun 1538 |
| Edinburgh, Scotland | |
| D: | 11 Jun 1560 |
| Edinburgh, Midlothian, Scotland | |
| Margaret Tudor 1489-1541 |
| Margaret Tudor | |
| B: | 29 Nov 1489 |
| Westminster, London, England | |
| D: | 18 Oct 1541 |
| Methven, Perthshire, Scotland | |
| James (James IV) Stewart 1473-1513 |
| James (James IV) Stewart | |
| B: | 17 Mar 1473 |
| Edinburgh, Midlothian, Scotland | |
| M: | 8 Aug 1503 |
| Edinburgh, Midlothian, Scotland | |
| D: | 9 Sep 1513 |
| Flodden Field, Branxton, Northumberland, England | |
| Henry (Henry VII) Tudor 1457-1509 |
| Henry (Henry VII) Tudor | |
| B: | 28 Jan 1457 |
| Pembroke Castle, Pembrokeshire, England | |
| D: | 21 Apr 1509 |
| Richmond Palace, Eichmond, Surrey, England | |
| Elizabeth York 1466-1503 |
| Elizabeth York | |
| B: | 11 Feb 1466 |
| Palace of Westminster, London, Middlesex, England | |
| M: | 18 Jan 1486 |
| Westminster, London, England | |
| D: | 11 Feb 1503 |
| Tower of London, London, Middlesex, England | |
|
| Margaret Beaufort 1443-1509 |
| Margaret Beaufort | |
| B: | 31 May 1443 |
| Bletsoe Castle, Bedfordshire | |
| D: | 29 Jun 1509 |
| Edmund Tudor 1430-1456 |
| Edmund Tudor | |
| B: | 11 Jun 1430 |
| D: | 3 Nov 1456 |
| St. David's Cathedral | |
